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KMID : 0368420030460020090
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Journal of Plant Biology
2003 Volume.46 No. 2 p.90 ~ p.94
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Assessing Competence for Adventitious Shoot Formation in Hypocotyl-Explant Cultures from Catharanthus roseus Cultivars
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Choi, Pil-Son
Lee, Soo-Young/Chug, Hwa-Jee/In, Dong-Soo/Choi, Dong-Wook/Liu, Jang Ryol
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Abstract
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Hypocotyl explants from 22 cultivars of Catharanthus roseus were cultured on various shoot-inducing media to assess their competence for adventitious shoot formation. The Murashige and Skoog (MS) media had been supplemented with 14 μM zeatin and 2.5 μM indole-3-butyric acid (IBA), 4.5 μM BA and 0.5 μM a-naphthaleneacetic acid (NAA), or 14 μM thidiazuron and 2.5 μM IBA. After eight weeks, the explants from ´Cooler Raspberry Red´ showed the greatest frequency of adventitious shoot formation, followed by ´Cooler Orchid´ and ´Cooler Treated´. The highest frequency (86.7%) for ´Cooler Raspberry Red´ was attained on the mediμM enhanced with 14 μM zeatin and 2.5 μM NAA. Excised adventitious shoots were then readily rooted on a half-strength MS basal mediμM. Afterward, the regenerated plantlets were transferred to potting soil and grown to maturity in a greenhouse.
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